Inhibition of simian virus 40 replication by kanamycin derivative.
نویسندگان
چکیده
منابع مشابه
Inhibition of simian virus 40 replication by kanamycin derivative.
Recently, the antiviral activity of kanamycin derivatives against influenza and herpes simplex virus has been reported1~3). The mechanism of antiviral action of these agents is not well understood. In this report, the mechanism of antiviral effect of 1-N-eicosanoyl-3"-N-(trifluoroacetyl)kanamycin (ETK), one of these agents, was studied. We have tried to test the effect of ETK on simian virus 40...
متن کاملInhibition of simian virus 40 DNA replication by specific modification of T-antigen with oxidized ATP.
We covalently bound periodate-oxidized ATP (oATP) to purified simian virus 40 (SV40) large T-antigen and determined the effect of this modification on viral DNA replication and three other biochemical activities of T-antigen. The oATP bound specifically to T-antigen, inhibiting the ATPase activity and preventing T-antigen from activating SV40 DNA replication in vitro. In contrast, binding of oA...
متن کاملRelationship of replication and transcription of Simian Virus 40 DNA.
RNA produced by the Simian Virus 40 (SV40) mutant tsA30 during lytic infection of kidney cells of African green monkeys was examined by RNA-DNA competition-hybridization. This mutant is temperature-sensitive in a function (gene A) that regulates synthesis of viral DNA. No detectable difference between mutant RNA synthesized at the permissive temperature (33 degrees ) and wild-type viral RNA was...
متن کاملOrigin and direction of simian virus 40 deoxyribonucleic acid replication.
Double-branched, circular, replicating deoxyribonucleic acid (DNA) molecules of simian virus 40 (SV40) have been cleaved by the R(1) restriction endonuclease from Escherichia coli. This enzyme introduces one double-strand break in SV40 DNA, at a specific site. The site of cleavage in the replicating molecules was used in this study to position the origin and the two branch points. Radioactively...
متن کاملUnwinding of parental strands during simian virus 40 DNA replication.
Pools of young (less than 60% replicated) and mature (60-90% replicated) replicating molecules of simian virus 40 (SV40) DNA have been treated at pH 12.2 in order to dissociate growing chains from the parental strands. The molecules are neutralized so that the parental strands can reassociate and they have then been isolated. They are covalently closed structures which sediment rapidly in alkal...
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ژورنال
عنوان ژورنال: The Journal of Antibiotics
سال: 1986
ISSN: 0021-8820,1881-1469
DOI: 10.7164/antibiotics.39.1769